Journal: Cancer immunology, immunotherapy : CII
Article Title: Tissue-resident memory CD103+CD8+ T cells in colorectal cancer: its implication as a prognostic and predictive liver metastasis biomarker.
doi: 10.1007/s00262-024-03709-2
Figure Lengend Snippet: Fig. 3 The expression of CD31 and α-SMA in clinical samples and the anti-angiogenic therapy promotes infiltration of CD103+CD8+ TRMs in tumor. A The expression of CD31 and α-SMA in CRC and CRC liver metastasis tissue. B The infiltra- tion level of CD103+CD8+ TRMs in the α-SMA high and low groups. C Comparison of median number and interquar- tile range of CD103+CD8+ TRMs. D The relationship of CD103+CD8+ TRMs and α-SMA+ vessel. E–F The infil- tration level of CD103+CD8+ TRMs in CRC patients who responded and non-respond to bevacizumab treatment. G Average tumor growth curves showing tumor volume in mice treated with either control or the Bevacizumab (n = 5 mice per group). H–K Representative flow cytometry plots (H) and the percentage of CD103+CD8+ TRMs (I) and representative flow cytometry plots (J) and the percentage of IFN-γ+ CD103+CD8+ TRMs (K) isolated from mice on day 21 (n = 5 mice per group). (Immunofluorescent staining, × 400, The white triangle in the tissue is CD103+CD8+ TRMs). CRC, colorectal cancer; TRM, tissue-resident memory T cell. α-SMA, alpha-smooth muscle actin. *p < 0.05; **p < 0.01; ***p < 0.001
Article Snippet: And 1 μl of fluorescein isothiocyanate (FITC) anti-mouse CD8 (cat. 11-0081-81, eBioScience), phycoerythrin (PE) anti-mouse CD103 (cat. E-AB-F1090D, Elabscience), and APC anti-mouse IFN-γ (cat. E-AB-F1101UE, Elabscience) were added to the tube, respectively.
Techniques: Expressing, Comparison, Control, Flow Cytometry, Isolation, Staining